Researchers at Kanazawa University report in the Journal of the American Chemical Society that in reactive host–guest systems, both the molecular recognition and the chemical reaction can happen first. Moreover, it is possible to make the order dependent on the guest ion, a discovery that may be relevant for drug-delivery applications.
Sometimes a molecule can only undergo a particular chemical reaction if it forms a so-called host–guest complex together with another molecule — the two molecules are then bound together not by covalent bonds but by intermolecular forces. What happens is that first, the host recognizes the guest, after which it can chemically react and become another molecule. But now, Shigehisa Akine and colleagues from Kanazawa University have shown that the reversed order is also possible: first, the host undergoes a chemical reaction, after which it recognizes and forms a complex with the guest ion. Moreover, they found that the order of recognition and reaction can be switched by modifying the guest ion. Distinguishing between the two alternatives (‘recognition first’ or ‘reaction first’) becomes important when the timescale on which the two processes happen differ significantly, a situation that could be exploited in applications including drug delivery.
For their study, the researchers used a cobalt-containing host molecule (a ‘metallohost’), which has a cavity that can accommodate a particular ion (charged atom) as a guest. The metallohost can undergo reactions of the type known as ligand exchange reaction. The advantage of using this host system is that the occurring reaction processes are slow, and easily monitorable by nuclear magnetic resonance (NMR) spectroscopy. As guest ion, Akine and colleagues used a compound named NaOTf, containing a sodium ion, which can occupy the host’s cavity when forming the host–guest complex.
After adding NaOTf to the metallohost, the NMR signal did not initially point to a structural change. However, after 3 hours, a change did occur, indicating the formation of new molecules. To determine whether the process was ‘recognition first’ or ‘reaction first’, the researchers examined the kinetics of the ligand exchange reaction, and its relation to the sodium concentration. They found that the reaction speed increased significantly with increasing the concentration of sodium, which made them conclude that for sodium the mechanism was ‘recognition first’.
Akine and colleagues performed similar experiments with potassium- and rubidium-based guest compounds. Interestingly, they observed that the ligand exchange then occurred in the guest-free form, meaning that the overall process was ‘reaction first’.
The observed dependence of the type of binding taking place on the type of guest metal ion not only add new insights into host-guest chemistry and their dynamics, but may also lead to applications. The scientists believe that “the understanding of the mechanism would help in developing new time-programmable guest uptake/release systems such as drug delivery systems.”
Illustration of the ‘reaction first’ and ‘recognition first’ scenarios in reactive host–guest systems (left) and the host molecule and its schematic representation used by Akine and colleagues (right).
Supplementary cover of J. Am. Chem. Soc. 10.1021/jacs.9b06926 showing the research by Shigehisa Akine et al. that is published in this issue.
Host–guest complexes are supramolecular structures consisting of two or more molecules or ions bound together by forces other than covalent bonds — the usual chemical bonds in which two atoms share a pair of electrons. Typical non-covalent bonds are van der Waals interactions (attraction between neutral atoms or molecules), hydrogen bonds (electrostatic attraction between a hydrogen atom in one molecule and an electronegative atom with a lone electron pair in another) and ionic bonds (electrostatic attraction between oppositely charged ions). Host–guest compounds can exist because of molecular recognition; the geometries and compositions of particular parts (moieties) of two nearby molecules are then such that a stable supramolecular structure develops, which is held in place through non-covalent bonds.
The research team of Shigehisa Akine at Kanazawa University investigated host–guest complexes undergoing reactions; they looked at whether recognition or reaction takes place first. They found that it is possible to switch between ‘recognition first’ and ‘reaction first’ by changing a guest metal ion.
Nuclear magnetic resonance
Nuclear magnetic resonance (NMR) spectroscopy is a technique capable of measuring local magnetic fields in the vicinity of atomic nuclei. The underlying principle is that when a molecule is placed in a magnetic field, an NMR signal is produced by the excitation of nuclei with radio waves, which is detected with sensitive radio receivers. The precise resonance radio frequency depends on the resonating atom’s environment in the molecule, thus providing information on the molecule’s structure. NMR is an important technique for studying host–guest complexes, as the (non-covalent) binding of the host and guest molecules causes a change in their electronic environments, leading to shifted signals in the NMR spectrum. Akine and colleagues used NMR to distinguish ‘reaction first’ and ‘recognition first’ pathways in reactive host–guest systems.
Title: Switching of Recognition First and Reaction First Mechanisms in Host–Guest Binding Associated with Chemical Reactions
Journal: Journal of the American Chemical Society
This work was supported in part by JSPS KAKENHI (Grant Numbers JP16H06510 (Coordination Asymmetry), JP26288022, and JP18H03913), Hokuriku Bank Foundation, The Kyoto Technoscience Center, Kanazawa University CHOZEN Project, and the World Premier International Research Initiative (WPI), MEXT, Japan.