WPI Nano Life Science Institute, Kanazawa University

Call for Bio-SPM Collaborative Research Proposal

The Nano Life Science Institute (NanoLSI), Institute for Frontier Science Initiative, Kanazawa University, is calling for applications for Bio-SPMs collaborative research.

1. Aims

The research teams in Kanazawa University have engaged in the pioneering development of original Bio-SPM technologies, including Super-resolution AFM (FM-AFM/3D-AFM), High-speed AFM, and Scanning Ion Conductance Microscope (SICM), and applied them to the life sciences.
WPI-NanoLSI, Kanazawa University, aims to contribute to the development of the nano life sciences by promoting collaborative research using these Bio-SPMs, beyond the boundaries of individual universities and research institutions. Here, we call for applications for collaborative research projects that are carried out by the applicants using our Bio-SPMs, with the cooperation of the NanoLSI faculty members.

* Please refer to the overview of each Bio-SPM technology at the end of this page.

2. Application Eligibility

Applicants must be researchers or engineers from national public or private universities, national
research institutes, private companies, overseas research institutes, etc. Depending on the applicant’s
affiliated institution, a collaborative research contract would be required.

3. Research Period

From the date of approval to March 31, 2019.

4. How to Apply

Prepare the following application materials (i.e., Form 1, Form 2, and curriculum vitae). If applicants
have discussed research topics, research schedule, schedule for visiting NanoLSI, etc. with the NanoLSI
faculty member(s), indicate these things in Form 1.

[Application Materials]

(Form 1) “2018 Academic Year Bio-SPMs Collaborative Research, Application and Collaborative Researcher Approval Form”  (PDF)    (WORD)

(Arbitrary Format) “Curriculum vitae for the principal investigator (applicant) only”

 

[Submission Deadline]

Applications must reach us by
1st in 2018: May 11, 2018 (Friday), 17:00 JST  Over
2nd in 2018: July 31, 2018 (Tuesday), 17:00 JST Over
3rd in 2018: October 12, 2018 (Friday), 17:00 JST
4th in 2018: November 30, 2018 (Friday), 17:00 JST

1st in 2019 will be May 10, 2018 (Friday), 17:00 JST

For urgent cases, applications will be accepted regardless of the deadline. In such cases, contact the person in charge of “5. Inquiries Regarding Research Contents.”

[Submission Destination]

Nano Life Science Institute, Kanazawa University,
Kakuma-Machi, Kanazawa 920-1192, Japan.
Tel.: +81-76-234-4574, Fax: +81-76-234-4569
E-mail: kunioka@staff.kanazawa-u.ac.jp

5. Inquiries Regarding Research Contents

Send an inquiry to the e-mail address below. The inquiry should include the type of Bio-SPMs you wish to use (either Super-resolution AFM [FM-AFM/3D-AFM], High-speed AFM, or SICM). The reply will come from the person in charge of the specific Bio-SPM technology.
E-mail: kunioka@staff.kanazawa-u.ac.jp

6. Decisions

After the review by the Expert Committee of NanoLSI, Kanazawa University, the director of NanoLSI will decide which applications will be approved. The decision will be informed to the applicants.

7. Research Report

An approved researcher has an obligation to report the research results when the research period is over. Using Form 3 and 4, prepare and submit the research report. The deadline is May 10, 2019. The summary of research results (Form 4) will be publicly posted on the NanoLSI Bio-SPMs Collaborative Research website in the following academic year.

(Form 3) “2018 Academic Year Bio-SPMs Collaborative Research, Research Report”  (PDF)   (WORD)

(Form 4) “2018 Academic Year Bio-SPMs Collaborative Research, Research Report Summary”  (PDF)   (WORD)

8. When the research papers are published

In order to measure the effectiveness of this Bio-SPMs collaborative research activity, we would like to collect the data of research papers published through collaborative research carried out at NanoLSI. Therefore, we sincerely hope that the approved researchers will kindly inform us when the research papers are published. In addition, for three years after the research period is over, we would like to ask all the approved researchers every June via e-mail if the research papers through collaborative research were newly published.

9. Others

(a) Research applications that match our policy described in “1. Aims” will be preferentially approved. It is preferable that applicants have already conducted sample preparations and preliminary
experiments, for example, biochemical experiments, test of observation conditions, and microscopic analysis by optical microscope, electron microscope, SPM, etc. However, applications without any preliminary results will also be approved depending on the contents.

(b) When a submitted application is approved, the principal investigator (PI) responsible for the collaborative research should be a collaborative researcher of NanoLSI, Kanazawa University. All
members who participate in the approved research project can stay at Kakuma Guest House (http://guesthouse.w3.kanazawa-u.ac.jp/index.html). If no room is available at Kakuma Guest House,
stay at hotels in downtown.

(c) If you cannot cover the travel and accommodation expenses for staying at Kanazawa University, mention it in the appropriate part of Form 1. Travel and accommodation expenses will be supported up to 200K JPY, after the review by the Expert Committee of NanoLSI.

(d) For the outstanding proposals related to cancer research, travel and accommodation expense will be supported up to 200K JPY by Cancer Research Institute, Kanazawa University. Adopted should mention as follows when the results is published, “This work was partly supported by Extramural Collaborative Research Grant of Cancer Research Institute, Kanazawa University.”

(e) Graduate and undergraduate students can also participate in a collaborative research team. In such cases, approval from their supervisor is absolutely necessary. The approval form is available in Form 1: “2018 Academic Year Bio-SPMs Collaborative Research, Application and Collaborative Researcher Approval Form.” Furthermore, students must take the Personal Accident Insurance for Students Pursuing Education and Research. Travel and accommodation expenses can be covered for only graduate students, but not for undergraduate students.

(f) If the supervisor of the student collaborative researchers changes, a new approval from the new supervisor would be necessary. In such cases, contact the person in charge of the Bio-SPMs
Collaborative Research (E-mail: kunioka@staff.kanazawa-u.ac.jp).

 

[Supplementary Information]

  • Regarding the Birth date, Age, and Gender fields in the Application
    These fields are necessary for making statistics for the evaluation report by the Ministry of Education, Culture, Sports, Science and Technology, in which the ages and genders of collaborative researchers must be presented. The information provided will not influence any decisions. In addition, personal information provided will be handled with the utmost caution.
  • Regarding Filling out the Application
    Add more spaces freely if not enough space is available in the application form. The number of pages can be increased.

 

[Over view of each Bio-SPM technology]

  • Super-resolution AFM (FM-AFM & 3D-AFM)

FM-AFM (Frequency-modulation Atomic Force Microscope)can visualize subnanometer-scale surface structures of biomolecules in solution. Combined with 3D scanning technique, it can also visualize 3D distribution of hydration and flexible surface structures at solid-liquid interfaces. The imaging rate of FM-AFM and 3D-AFM is typically 1 min/frame. The optimal spatial resolution of the instrument is 0.3 nm in the lateral direction and 0.01 nm in the vertical direction. In the case of biomolecular imaging, the practical resolution is mostly determined by the fluctuation of the surface structures rather than the instruments. For more details, see the following articles:

  1. H. Asakawa, S. Yoshioka, K. Nishimura, T. Fukuma, “Spatial Distribution of Lipid Headgroups and Water Molecules at Membrane/Water Interfaces Visualized by Three-Dimensional Scanning Force Microscopy”, ACS Nano 6, 9013-9020 (2012).
  2. H. Asakawa, K. Ikegami, M. Setou, N. Watanabe, M. Tsukada, T. Fukuma, “Submolecular-Scale Imaging of α-Helices and C-Terminal Domains of Tubulins by Frequency Modulation Atomic Force Microscopy in Liquid”, Biophys. J. 101, 1270-1276 (2011).
  3. T. Fukuma, “Water distribution at solid/liquid interfaces visualized by frequency modulation atomic force microscopy”, Sci. Technol. Adv. Mater. 11, 033003 (18 pages) (2010).

 

  • High-speed AFM (HS-AFM)

HS-AFM (High-speed Atomic Force Microscope) can visualize moving objects in solution. Its temporal resolution is typically 100 ms/frame, while the spatial resolution is 2-3 nm in the lateral direction and 0.15 nm in the vertical direction. When it is applied to protein molecules in action, the acquired HS-AFM images can provide a significant insight into how the molecules function. For more details, see the following review articles:

  1. T. Ando, T. Uchihashi, S. Scheuring, “Filming biomolecular processes by high-speed atomic force microscopy”, Chem. Rev. 114, 3120-3188 (2014).
  2. T. Ando, T. Uchihashi, N. Kodera, “High-speed AFM and applications to biomolecular systems”, Annu. Rev. Biophys. 42, 393-414 (2013).
  3. T. Uchihashi, N. Kodera, T. Ando, “Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy”, Nature Protocols 7, 1193-1206 (2012).

    

  • Scanning Ion Conductance Microscopy (SICM)

SICM has a unique measurement principle and provides unprecedented opportunity that enables submicroscale functional imaging of single live cells by a combination of nanoscale local stimulation and noncontact topography imaging. The imaging rate of SICM is 30-300 s/frame. Spatial resolution of the instrument is 10 nm in the lateral direction and 5 nm in the vertical direction. For more details, see the following articles:

  1. P. Novak, C. Li, A. I. Shevchuk, R. Stepanyan, M. Caldwell, S. Hughes, T. G. Smart, J. Gorelik, V. P. Ostanin, M. J. Lab, G. W. J. Moss, G. I. Frolenkov, D. Klenerman, and Y. E. Korchev, “Nanoscale live-cell imaging using hopping probe ion conductance microscopy”, Nat. Methods 6, 279-281 (2009).
  2. V. O. Nikolaev, A. Moshkov, A. R. Lyon, M. Miragoli, P. Novak, H. Paur, M. J. Lohse, Y. E. Korchev, S. E. Harding, and J. Gorelik, “beta(2)-Adrenergic Receptor Redistribution in Heart Failure Changes cAMP Compartmentation”, Science 327, 1653-1657 (2010).
  3. Y. Zhou, M. Saito, T. Miyamoto, P. Novak, A. Shevchuk, Y. Korchev, T. Fukuma, Y. Takahashi, “Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy,” Anal. Chem. 90, 2891-2895 (2018).

 

[Application Materials]

Application Guidelines

(Form 1) “2018 Academic Year Bio-SPMs Collaborative Research, Application and Collaborative Researcher Approval Form”  (PDF)    (WORD)

 

※An approved researcher has an obligation to report the research results when the research period is over. The deadline is May 10, 2019.

(Form 3) “2018 Academic Year Bio-SPMs Collaborative Research, Research Report”   (PDF)   (WORD)

(Form 4) “2018 Academic Year Bio-SPMs Collaborative Research, Research Report Summary”   (PDF)   (WORD)

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